fla-5100 imager fluorescence analyzer Search Results


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Sysmex Corporation platelet clumps (clp) flag
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Raytest GmbH fla-5100 phosphorimager
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FUJIFILM life fla-5100 imaging system
Life Fla 5100 Imaging System, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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BPS Bioscience ezh2 enzyme complex
Ezh2 Enzyme Complex, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Sysmex Corporation xe-5000
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BPS Bioscience recombinant human ezh2 proteins
Weaver syndrome mutants are impaired in their histone methyltransferase activity in vitro . Histone methyltransferase reactions were performed using 2 μg purified core histones and 0.67 μM 3 H‐S‐adenosyl‐methionine ( 3 H‐SAM). Each reaction was incubated with 250 ng of either wild‐type (WT) or a mutant HMTase complex (or no enzyme controls). Histone methyltransferase activity was measured based on the incorporation of 3 H‐labeled methyl groups, represented in scintillation counts per minute. Counts were normalized by subtracting background counts (i.e., no enzyme) from the total counts. A : Incorporation of tritiated methyl groups from 3 H‐SAM onto core histones is shown for each complex: <t>EZH2</t> WT • , p.(Phe672Ile) × , p.(Pro132Ser) ★ , p.(Tyr153del) △, p.(His694Tyr) ▽, p.(Glu745Lys) ▴, p.(Ala682Thr) ▾, p.(Arg684Cys) ▪, p.(Tyr133Cys) □, and p.(Asp185His) ◇. Error bars represent standard deviation (SD) within the groups “EZH2 WT” and “EZH2 mutants.” Unpaired t‐test showed statistically significant difference between the two groups (P value < 0.0001). B : Incorporation of tritiated methyl groups from 3 H‐SAM onto core histones is shown for the positive control EZH2 WT, the negative control EZH2 (p.Phe672Ile), and the mutant complex with activity closest to WT, namely, EZH2 (p.Pro132Ser). Error bars represent SD of four independent replicates for the controls, and three independent replicates for the mutant EZH2 (p.Pro132Ser). One‐way ANOVA showed statistically significant difference between all groups (overall P value < 0.0001; P values between WT and p.(Phe672Ile), between p.(Phe672Ile) and p.(Pro132Ser), and between WT and p.(Pro132Ser) were all <0.05).
Recombinant Human Ezh2 Proteins, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Sysmex Corporation xn modular analyzer
Weaver syndrome mutants are impaired in their histone methyltransferase activity in vitro . Histone methyltransferase reactions were performed using 2 μg purified core histones and 0.67 μM 3 H‐S‐adenosyl‐methionine ( 3 H‐SAM). Each reaction was incubated with 250 ng of either wild‐type (WT) or a mutant HMTase complex (or no enzyme controls). Histone methyltransferase activity was measured based on the incorporation of 3 H‐labeled methyl groups, represented in scintillation counts per minute. Counts were normalized by subtracting background counts (i.e., no enzyme) from the total counts. A : Incorporation of tritiated methyl groups from 3 H‐SAM onto core histones is shown for each complex: <t>EZH2</t> WT • , p.(Phe672Ile) × , p.(Pro132Ser) ★ , p.(Tyr153del) △, p.(His694Tyr) ▽, p.(Glu745Lys) ▴, p.(Ala682Thr) ▾, p.(Arg684Cys) ▪, p.(Tyr133Cys) □, and p.(Asp185His) ◇. Error bars represent standard deviation (SD) within the groups “EZH2 WT” and “EZH2 mutants.” Unpaired t‐test showed statistically significant difference between the two groups (P value < 0.0001). B : Incorporation of tritiated methyl groups from 3 H‐SAM onto core histones is shown for the positive control EZH2 WT, the negative control EZH2 (p.Phe672Ile), and the mutant complex with activity closest to WT, namely, EZH2 (p.Pro132Ser). Error bars represent SD of four independent replicates for the controls, and three independent replicates for the mutant EZH2 (p.Pro132Ser). One‐way ANOVA showed statistically significant difference between all groups (overall P value < 0.0001; P values between WT and p.(Phe672Ile), between p.(Phe672Ile) and p.(Pro132Ser), and between WT and p.(Pro132Ser) were all <0.05).
Xn Modular Analyzer, supplied by Sysmex Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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FUJIFILM imaging plate for ̃16 hours fujifilm fla-5100 imaging system
Weaver syndrome mutants are impaired in their histone methyltransferase activity in vitro . Histone methyltransferase reactions were performed using 2 μg purified core histones and 0.67 μM 3 H‐S‐adenosyl‐methionine ( 3 H‐SAM). Each reaction was incubated with 250 ng of either wild‐type (WT) or a mutant HMTase complex (or no enzyme controls). Histone methyltransferase activity was measured based on the incorporation of 3 H‐labeled methyl groups, represented in scintillation counts per minute. Counts were normalized by subtracting background counts (i.e., no enzyme) from the total counts. A : Incorporation of tritiated methyl groups from 3 H‐SAM onto core histones is shown for each complex: <t>EZH2</t> WT • , p.(Phe672Ile) × , p.(Pro132Ser) ★ , p.(Tyr153del) △, p.(His694Tyr) ▽, p.(Glu745Lys) ▴, p.(Ala682Thr) ▾, p.(Arg684Cys) ▪, p.(Tyr133Cys) □, and p.(Asp185His) ◇. Error bars represent standard deviation (SD) within the groups “EZH2 WT” and “EZH2 mutants.” Unpaired t‐test showed statistically significant difference between the two groups (P value < 0.0001). B : Incorporation of tritiated methyl groups from 3 H‐SAM onto core histones is shown for the positive control EZH2 WT, the negative control EZH2 (p.Phe672Ile), and the mutant complex with activity closest to WT, namely, EZH2 (p.Pro132Ser). Error bars represent SD of four independent replicates for the controls, and three independent replicates for the mutant EZH2 (p.Pro132Ser). One‐way ANOVA showed statistically significant difference between all groups (overall P value < 0.0001; P values between WT and p.(Phe672Ile), between p.(Phe672Ile) and p.(Pro132Ser), and between WT and p.(Pro132Ser) were all <0.05).
Imaging Plate For ̃16 Hours Fujifilm Fla 5100 Imaging System, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
TaKaRa fla5100
Weaver syndrome mutants are impaired in their histone methyltransferase activity in vitro . Histone methyltransferase reactions were performed using 2 μg purified core histones and 0.67 μM 3 H‐S‐adenosyl‐methionine ( 3 H‐SAM). Each reaction was incubated with 250 ng of either wild‐type (WT) or a mutant HMTase complex (or no enzyme controls). Histone methyltransferase activity was measured based on the incorporation of 3 H‐labeled methyl groups, represented in scintillation counts per minute. Counts were normalized by subtracting background counts (i.e., no enzyme) from the total counts. A : Incorporation of tritiated methyl groups from 3 H‐SAM onto core histones is shown for each complex: <t>EZH2</t> WT • , p.(Phe672Ile) × , p.(Pro132Ser) ★ , p.(Tyr153del) △, p.(His694Tyr) ▽, p.(Glu745Lys) ▴, p.(Ala682Thr) ▾, p.(Arg684Cys) ▪, p.(Tyr133Cys) □, and p.(Asp185His) ◇. Error bars represent standard deviation (SD) within the groups “EZH2 WT” and “EZH2 mutants.” Unpaired t‐test showed statistically significant difference between the two groups (P value < 0.0001). B : Incorporation of tritiated methyl groups from 3 H‐SAM onto core histones is shown for the positive control EZH2 WT, the negative control EZH2 (p.Phe672Ile), and the mutant complex with activity closest to WT, namely, EZH2 (p.Pro132Ser). Error bars represent SD of four independent replicates for the controls, and three independent replicates for the mutant EZH2 (p.Pro132Ser). One‐way ANOVA showed statistically significant difference between all groups (overall P value < 0.0001; P values between WT and p.(Phe672Ile), between p.(Phe672Ile) and p.(Pro132Ser), and between WT and p.(Pro132Ser) were all <0.05).
Fla5100, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Sysmex Corporation olo® system
Regression analysis in the newborn population. Results of the method comparison study between the Sight <t>OLO®</t> and <t>the</t> <t>Sysmex</t> XN™ hematology analyzers. Graphs indicate Pearson correlation, slope and intercept for the main parameters. WBC: White Blood Cells, RBC: Red Blood Cells, PLT: Platelets, HGB: Hemoglobin, NEUT: Neutrophils, LYMPHO: Lymphocytes, MONO: Monocytes, EOS: Eosinophils, BASO: Basophils.
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FUJIFILM aida software
Regression analysis in the newborn population. Results of the method comparison study between the Sight <t>OLO®</t> and <t>the</t> <t>Sysmex</t> XN™ hematology analyzers. Graphs indicate Pearson correlation, slope and intercept for the main parameters. WBC: White Blood Cells, RBC: Red Blood Cells, PLT: Platelets, HGB: Hemoglobin, NEUT: Neutrophils, LYMPHO: Lymphocytes, MONO: Monocytes, EOS: Eosinophils, BASO: Basophils.
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Sysmex Corporation sysmex analyzer plt-f
Regression analysis in the newborn population. Results of the method comparison study between the Sight <t>OLO®</t> and <t>the</t> <t>Sysmex</t> XN™ hematology analyzers. Graphs indicate Pearson correlation, slope and intercept for the main parameters. WBC: White Blood Cells, RBC: Red Blood Cells, PLT: Platelets, HGB: Hemoglobin, NEUT: Neutrophils, LYMPHO: Lymphocytes, MONO: Monocytes, EOS: Eosinophils, BASO: Basophils.
Sysmex Analyzer Plt F, supplied by Sysmex Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Weaver syndrome mutants are impaired in their histone methyltransferase activity in vitro . Histone methyltransferase reactions were performed using 2 μg purified core histones and 0.67 μM 3 H‐S‐adenosyl‐methionine ( 3 H‐SAM). Each reaction was incubated with 250 ng of either wild‐type (WT) or a mutant HMTase complex (or no enzyme controls). Histone methyltransferase activity was measured based on the incorporation of 3 H‐labeled methyl groups, represented in scintillation counts per minute. Counts were normalized by subtracting background counts (i.e., no enzyme) from the total counts. A : Incorporation of tritiated methyl groups from 3 H‐SAM onto core histones is shown for each complex: EZH2 WT • , p.(Phe672Ile) × , p.(Pro132Ser) ★ , p.(Tyr153del) △, p.(His694Tyr) ▽, p.(Glu745Lys) ▴, p.(Ala682Thr) ▾, p.(Arg684Cys) ▪, p.(Tyr133Cys) □, and p.(Asp185His) ◇. Error bars represent standard deviation (SD) within the groups “EZH2 WT” and “EZH2 mutants.” Unpaired t‐test showed statistically significant difference between the two groups (P value < 0.0001). B : Incorporation of tritiated methyl groups from 3 H‐SAM onto core histones is shown for the positive control EZH2 WT, the negative control EZH2 (p.Phe672Ile), and the mutant complex with activity closest to WT, namely, EZH2 (p.Pro132Ser). Error bars represent SD of four independent replicates for the controls, and three independent replicates for the mutant EZH2 (p.Pro132Ser). One‐way ANOVA showed statistically significant difference between all groups (overall P value < 0.0001; P values between WT and p.(Phe672Ile), between p.(Phe672Ile) and p.(Pro132Ser), and between WT and p.(Pro132Ser) were all <0.05).

Journal: Human Mutation

Article Title: Weaver Syndrome‐Associated EZH2 Protein Variants Show Impaired Histone Methyltransferase Function In Vitro

doi: 10.1002/humu.22946

Figure Lengend Snippet: Weaver syndrome mutants are impaired in their histone methyltransferase activity in vitro . Histone methyltransferase reactions were performed using 2 μg purified core histones and 0.67 μM 3 H‐S‐adenosyl‐methionine ( 3 H‐SAM). Each reaction was incubated with 250 ng of either wild‐type (WT) or a mutant HMTase complex (or no enzyme controls). Histone methyltransferase activity was measured based on the incorporation of 3 H‐labeled methyl groups, represented in scintillation counts per minute. Counts were normalized by subtracting background counts (i.e., no enzyme) from the total counts. A : Incorporation of tritiated methyl groups from 3 H‐SAM onto core histones is shown for each complex: EZH2 WT • , p.(Phe672Ile) × , p.(Pro132Ser) ★ , p.(Tyr153del) △, p.(His694Tyr) ▽, p.(Glu745Lys) ▴, p.(Ala682Thr) ▾, p.(Arg684Cys) ▪, p.(Tyr133Cys) □, and p.(Asp185His) ◇. Error bars represent standard deviation (SD) within the groups “EZH2 WT” and “EZH2 mutants.” Unpaired t‐test showed statistically significant difference between the two groups (P value < 0.0001). B : Incorporation of tritiated methyl groups from 3 H‐SAM onto core histones is shown for the positive control EZH2 WT, the negative control EZH2 (p.Phe672Ile), and the mutant complex with activity closest to WT, namely, EZH2 (p.Pro132Ser). Error bars represent SD of four independent replicates for the controls, and three independent replicates for the mutant EZH2 (p.Pro132Ser). One‐way ANOVA showed statistically significant difference between all groups (overall P value < 0.0001; P values between WT and p.(Phe672Ile), between p.(Phe672Ile) and p.(Pro132Ser), and between WT and p.(Pro132Ser) were all <0.05).

Article Snippet: To test our hypothesis, we designed recombinant human EZH2 proteins, had them preassembled into PRC2 complexes (BPS Bioscience, San Diego CA), and tested their activity in vitro using a well‐accepted in vitro assay [Ernst et al., ; Yap et al., ; Score et al., ].

Techniques: Activity Assay, In Vitro, Purification, Incubation, Mutagenesis, Labeling, Standard Deviation, Positive Control, Negative Control

Regression analysis in the newborn population. Results of the method comparison study between the Sight OLO® and the Sysmex XN™ hematology analyzers. Graphs indicate Pearson correlation, slope and intercept for the main parameters. WBC: White Blood Cells, RBC: Red Blood Cells, PLT: Platelets, HGB: Hemoglobin, NEUT: Neutrophils, LYMPHO: Lymphocytes, MONO: Monocytes, EOS: Eosinophils, BASO: Basophils.

Journal: Practical Laboratory Medicine

Article Title: Complete blood count in neonatal Intensive care Unit (NICU): Performance comparison between POCT Sight OLO® and Sysmex XN-9100™ hematology analyzers

doi: 10.1016/j.plabm.2025.e00453

Figure Lengend Snippet: Regression analysis in the newborn population. Results of the method comparison study between the Sight OLO® and the Sysmex XN™ hematology analyzers. Graphs indicate Pearson correlation, slope and intercept for the main parameters. WBC: White Blood Cells, RBC: Red Blood Cells, PLT: Platelets, HGB: Hemoglobin, NEUT: Neutrophils, LYMPHO: Lymphocytes, MONO: Monocytes, EOS: Eosinophils, BASO: Basophils.

Article Snippet: Flags of the OLO® system were in partial accordance with the Sysmex XNTM system, but the POCT showed good performance compared to the final report, after revision carried out by the laboratory specialist.

Techniques: Comparison

Bias plot in the newborn population. Bland Altman of the results of a method comparison study between the Sight OLO® and the Sysmex XN™ hematology analyzers for the main measurements in the newborn population. WBC: White Blood Cells, RBC: Red Blood Cells, PLT: Platelets, HGB: Hemoglobin, NEUT: Neutrophils, LYMPHO: Lymphocytes, MONO: Monocytes, EOS: Eosinophils, BASO: Basophils.

Journal: Practical Laboratory Medicine

Article Title: Complete blood count in neonatal Intensive care Unit (NICU): Performance comparison between POCT Sight OLO® and Sysmex XN-9100™ hematology analyzers

doi: 10.1016/j.plabm.2025.e00453

Figure Lengend Snippet: Bias plot in the newborn population. Bland Altman of the results of a method comparison study between the Sight OLO® and the Sysmex XN™ hematology analyzers for the main measurements in the newborn population. WBC: White Blood Cells, RBC: Red Blood Cells, PLT: Platelets, HGB: Hemoglobin, NEUT: Neutrophils, LYMPHO: Lymphocytes, MONO: Monocytes, EOS: Eosinophils, BASO: Basophils.

Article Snippet: Flags of the OLO® system were in partial accordance with the Sysmex XNTM system, but the POCT showed good performance compared to the final report, after revision carried out by the laboratory specialist.

Techniques: Comparison

Bias plot in the adult population. Bland Altman of the results of method comparison study between the Sight OLO® and the Sysmex XN™ hematology analyzers for the main measurands in the adult population. WBC: White Blood Cells, RBC: Red Blood Cells, PLT: Platelets, HGB: Hemoglobin, NEUT: Neutrophils, LYMPHO: Lymphocytes, MONO: Monocytes, EOS: Eosinophils, BASO: Basophils.

Journal: Practical Laboratory Medicine

Article Title: Complete blood count in neonatal Intensive care Unit (NICU): Performance comparison between POCT Sight OLO® and Sysmex XN-9100™ hematology analyzers

doi: 10.1016/j.plabm.2025.e00453

Figure Lengend Snippet: Bias plot in the adult population. Bland Altman of the results of method comparison study between the Sight OLO® and the Sysmex XN™ hematology analyzers for the main measurands in the adult population. WBC: White Blood Cells, RBC: Red Blood Cells, PLT: Platelets, HGB: Hemoglobin, NEUT: Neutrophils, LYMPHO: Lymphocytes, MONO: Monocytes, EOS: Eosinophils, BASO: Basophils.

Article Snippet: Flags of the OLO® system were in partial accordance with the Sysmex XNTM system, but the POCT showed good performance compared to the final report, after revision carried out by the laboratory specialist.

Techniques: Comparison

Instrumental analytical alarms (flags) concordance between Sight  OLO®  and  Sysmex  XN™ in the newborn population.

Journal: Practical Laboratory Medicine

Article Title: Complete blood count in neonatal Intensive care Unit (NICU): Performance comparison between POCT Sight OLO® and Sysmex XN-9100™ hematology analyzers

doi: 10.1016/j.plabm.2025.e00453

Figure Lengend Snippet: Instrumental analytical alarms (flags) concordance between Sight OLO® and Sysmex XN™ in the newborn population.

Article Snippet: Flags of the OLO® system were in partial accordance with the Sysmex XNTM system, but the POCT showed good performance compared to the final report, after revision carried out by the laboratory specialist.

Techniques:

Instrumental analytical alarms (flags) concordance between Sight  OLO®  and  Sysmex  XN™ in the adult population.

Journal: Practical Laboratory Medicine

Article Title: Complete blood count in neonatal Intensive care Unit (NICU): Performance comparison between POCT Sight OLO® and Sysmex XN-9100™ hematology analyzers

doi: 10.1016/j.plabm.2025.e00453

Figure Lengend Snippet: Instrumental analytical alarms (flags) concordance between Sight OLO® and Sysmex XN™ in the adult population.

Article Snippet: Flags of the OLO® system were in partial accordance with the Sysmex XNTM system, but the POCT showed good performance compared to the final report, after revision carried out by the laboratory specialist.

Techniques: